Holotype HLA

Holotype HLA provides deep and even coverage of the whole region, with balance between alleles at the same locus, between the loci within a sample and also between samples – up to 96 samples per Miseq run at 11 loci, up to 192 samples at 7 loci and 288 samples at 5 loci - enabling better phasing, reduced ambiguity, and optimal utilization of sequence output.

Holotype HLA is the most accurate and easy to use NGS-based HLA genotyping product. However, the further elimination of human and random error remains a desirable goal for any lab. To ensure reproducibility and repeatability among technical staff and to reduce the possibility of human error, pre-PCR and post-PCR steps of Holotype HLA can be automated on a variety of liquid handling robots. 

Holotype HLA comes in three configurations (5, 7 & 11 loci) and two sizes (24 & 96 samples). All kits are splittable to batch just the samples you need, and can also be mixed with Monotype HLA samples (HLA-B, HLA-DQ or custom). Certain liquid handling instruments also have the flexibility to mix up the numbers of samples and loci to match your workflow.