NanoType

NanoTYPE™ is a HLA typing assay using third generation sequencing technology from Oxford Nanopore Technologies (ONT). The kit contains all necessary reagents for the amplification of your sample and comes with our tailored genotyping software, NanoTYPER™. The kit has been designed to be combined with a rapid barcoding library preparation from ONT.

All 11 loci (HLA-A, B, C, DQA1, DQB1, DPA1, DPB1, DRB1 and DRB345) are amplified in a single long-range multiplex PCR. The user can choose between 2 different protocols, either for a single sample urgent typing or multiple samples.

The easy workflow, the rapid turnaround time, the long reads and the absence of capital investment for the instrument makes it an ideal primary or a complementary technique in your lab.

Nanopore-Based Typing

Oxford Nanopore has developed a new generation of sensing technology that uses nanopores embedded in high tech electronics to perform precise molecular analysis. When a DNA fragment is entering a nanopore, each DNA base is disrupting the electrical field with a specific signature and can be used as a single molecule detector. The deconvolution of the electrical signal is done using a basecaller converting the electric signal into a DNA sequence with a FASTQ output format This FASTQ file is then imported into NanoTYPER software for genotyping. One major advantage of nanopore sequencing is the long reads facilitating the phasing of even long-distant SNPs resulting in lower number of ambiguities.